| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129694 |
| Chromosome: | chromosome 1 |
| Location: | 5429053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038151 | (1 of 1) PTHR23069:SF0 - TAT-BINDING HOMOLOG 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTGTACCGTAAGGGTGAGGTTTGCAAA |
| Internal bar code: | ATGGGTAGTGTACGAGTAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 97.9959 |
| LEAP-Seq n confirming: | 1907 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCTGTGGATTCTTACG |
| Suggested primer 2: | ACCGTTATTTCGTTGTTCGC |