| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.129699 |
| Chromosome: | chromosome 5 |
| Location: | 1035978 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246377 | ADA2 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.2 - Adenine deaminase / Adenine aminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGCTCGGTGCTAAAACAGTGCCGGTGC |
| Internal bar code: | GTTGAGCGGTGCAGACAGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 98.8008 |
| LEAP-Seq n confirming: | 2554 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGAACACAATCCGAAAG |
| Suggested primer 2: | ATTAGCCGTTACAAATGCGG |