Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.129704 |
Chromosome: | chromosome_3 |
Location: | 3042423 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g164250 | FAP262 | Flagellar Associated Protein, protein kinase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGTGGTCCCGCAGCTTGGCCTGCGAGGCG |
Internal bar code: | CGTTCCGCATTATGACGCGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 639 |
LEAP-Seq percent confirming: | 99.7378 |
LEAP-Seq n confirming: | 2663 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGATATCCACCCAGCGTT |
Suggested primer 2: | GCTCACCATAAAGCTCTGGC |