| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.130915 |
| Chromosome: | chromosome 7 |
| Location: | 2130665 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326450 | VPS5B | (1 of 2) K17917 - sorting nexin-1/2 (SNX1_2); Subunit of Retromer complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCGCGTGCATGATGGGAGCGAGCAAGC |
| Internal bar code: | CTAGCAAGGCGTCATCCCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 99.8755 |
| LEAP-Seq n confirming: | 1604 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGTAGCAGCCATCAACG |
| Suggested primer 2: | CTGAAGTGGCGTTATGGGTT |