Insertion junction: LMJ.RY0402.131377_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.131377
Chromosome:chromosome 12
Location:7285822
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g557700 (1 of 1) K01069 - hydroxyacylglutathione hydrolase (E3.1.2.6, gloB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCAGTCGGGATGAGGCCAGCGACGTGC
Internal bar code:AAGGGCGCGGACGGCTGAAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:271
LEAP-Seq percent confirming:30.0055
LEAP-Seq n confirming:2735
LEAP-Seq n nonconfirming:6380
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAATGTATGGCAGGTGTG
Suggested primer 2:GTGTCACGAACAAGAGCGAA