Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.131475 |
Chromosome: | chromosome_4 |
Location: | 674846 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g217951 | LCI33 | Low-CO2-inducible protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TACTGAGTCTAAGGGATTCAAGGGAATTGA |
Internal bar code: | CGGGGTCCGGGGACCGCATTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 731 |
LEAP-Seq percent confirming: | 99.1532 |
LEAP-Seq n confirming: | 8313 |
LEAP-Seq n nonconfirming: | 71 |
LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGACAGCCTGGACAACAGAG |
Suggested primer 2: | CTACTTCAAGCAAGGGCCTG |