| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131479 |
| Chromosome: | chromosome 17 |
| Location: | 1360144 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g706050 | SRR17 | (1 of 1) IPR001190//IPR005515//IPR017448 - SRCR domain // Vitelline membrane outer layer protein I (VOMI) // SRCR-like domain; Possible cell surface glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCTGGTAAGGCGTGTCTGGTAGTTGGG |
| Internal bar code: | TTTTTTGGTTGATTTTGTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 99.4874 |
| LEAP-Seq n confirming: | 11256 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCAGCCTTCACCTAAGC |
| Suggested primer 2: | AGTTCTTCCGATACCCGCTT |