| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.131571 |
| Chromosome: | chromosome 3 |
| Location: | 3751565 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169850 | TSC1 | (1 of 1) PTHR10890:SF3 - CYSTEINE--TRNA LIGASE, CYTOPLASMIC; Cysteinyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGCGAGATGGCACGGCGCCCGCCGCGC |
| Internal bar code: | CCTAGGACGTACCATATGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 72.5204 |
| LEAP-Seq n confirming: | 2040 |
| LEAP-Seq n nonconfirming: | 773 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGAAGAAGCGGTCGTT |
| Suggested primer 2: | CCTTCTCCTTCCTCCAGCTT |