Mutant strain: LMJ.RY0402.131571

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.131571_1 Cre03.g169850 TSC1 (1 of 1) PTHR10890:SF3 - CYSTEINE--TRNA LIGASE, CYTOPLASMIC; Cysteinyl-tRNA synthetase intron 73
LMJ.RY0402.131571_2 Cre11.g467597 (1 of 1) IPR000104//IPR003439//IPR003593//IPR013525//IPR013581//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // Plant PDR ABC transporter associated // P-loop containing nucleoside triphosphate hydrolase CDS 95
LMJ.RY0402.131571_3 Cre11.g467597 (1 of 1) IPR000104//IPR003439//IPR003593//IPR013525//IPR013581//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // Plant PDR ABC transporter associated // P-loop containing nucleoside triphosphate hydrolase CDS 95
LMJ.RY0402.131571_4 Cre11.g467756 MMP5 Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) CDS 73

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Paromomycin, screen 5 of 6 (~10.0% growth defect)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.