| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.131571 |
| Chromosome: | chromosome 11 |
| Location: | 1559691 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467756 | MMP5 | Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGCAATCACCACCAAGTTGTAGTCTG |
| Internal bar code: | GAGTCGTCTAGTAGTTGCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 99.5419 |
| LEAP-Seq n confirming: | 3694 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTACCTCATGGCCGTGTC |
| Suggested primer 2: | TCCTACCCGGATACTTCACG |