| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132069 |
| Chromosome: | chromosome_10 |
| Location: | 2916511 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g440200 | SMC5B | Structural Maintenance of Chromosomes protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCACAGGCAAGGACGCGACCCAGAAAGAC |
| Internal bar code: | TCATGAGGGCGGTTGACTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 96.1783 |
| LEAP-Seq n confirming: | 151 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGGTCCTCTCTCATGCC |
| Suggested primer 2: | ACCTCGTGCTGGATTGAAAC |