| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132214 |
| Chromosome: | chromosome 12 |
| Location: | 7986477 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552050 | (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTCCCCGCGGATTTAGTGGGTTGATGC |
| Internal bar code: | TGCCACTACAGTTAATGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 99.7758 |
| LEAP-Seq n confirming: | 6230 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCTGAATGGATGGCTTG |
| Suggested primer 2: | CCTTGATGACCACCTGTGTG |