| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132332 |
| Chromosome: | chromosome 3 |
| Location: | 4638261 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177350 | (1 of 4) PF11891 - Domain of unknown function (DUF3411) (DUF3411) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCAACAGAGCACACAGCAAACAAGCCA |
| Internal bar code: | GTAGGTCTTTTATGCTTACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 283 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGCTGTAGCAGCCTAGC |
| Suggested primer 2: | GCAACTCAAGTCTCTTGCCC |