Insertion junction: LMJ.RY0402.132550_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132550
Chromosome:chromosome 6
Location:6347210
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g292000 (1 of 1) PF00790//PF03127 - VHS domain (VHS) // GAT domain (GAT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAACTCAGCCAGGGCTGCGATGCCGTAC
Internal bar code:AAGCGTAGACAGGGTGGTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:283
LEAP-Seq percent confirming:98.1073
LEAP-Seq n confirming:311
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAACTCACCTAATCGCTC
Suggested primer 2:CCAACTGGTCGTTGTTGCTA