| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132552 |
| Chromosome: | chromosome 7 |
| Location: | 2965161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332950 | SFA,SFA1 | (1 of 1) PF06705 - SF-assemblin/beta giardin (SF-assemblin); SF-assemblin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCCTAAGTTGGCCTTCCAAGCGTCCCT |
| Internal bar code: | GATGGGGCGGGCCCCGGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 99.5339 |
| LEAP-Seq n confirming: | 6833 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCTTGCTGTCTCCATGC |
| Suggested primer 2: | CTTGGAGCACATGAATCCCT |