| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133218 |
| Chromosome: | chromosome 3 |
| Location: | 6311137 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193400 | (1 of 2) PTHR30603 - RNA POLYMERASE SIGMA FACTOR RPO | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTACCAAACGTCGTGGGCAGCGCGGTC |
| Internal bar code: | CCGGTGACGTCATATTTTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 913 |
| LEAP-Seq percent confirming: | 99.5337 |
| LEAP-Seq n confirming: | 6617 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGTCGCCACCTCTACTC |
| Suggested primer 2: | GCCCTTCGCCTTAAAATACC |