| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133464 |
| Chromosome: | chromosome_1 |
| Location: | 7095616 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g051250 | DLC4,DLE1 | Flagellar outer dynein arm 18 kDa light chain LC4 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTCCTTGAACTCGATGCAGCGGCTGCCGT |
| Internal bar code: | GAACGGTGCTCGAGCTGCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 93.0801 |
| LEAP-Seq n confirming: | 2058 |
| LEAP-Seq n nonconfirming: | 153 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACAAGTGCGAAAAGATA |
| Suggested primer 2: | CTTTTGCTACGGGGACACAT |