Insertion junction: LMJ.RY0402.133502_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.133502
Chromosome:chromosome 4
Location:3789823
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g229550 SRR15 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTTGTCCGTTATCGTTGAACCGCCCTCA
Internal bar code:GGGCCGTAAGGAGAACGGCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:800
LEAP-Seq percent confirming:45.759
LEAP-Seq n confirming:25814
LEAP-Seq n nonconfirming:30599
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGAGGGCACGTGTTATTG
Suggested primer 2:AGTGTGGCCTCGATCAAATC