| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.133761 |
| Chromosome: | chromosome 13 |
| Location: | 2470042 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580000 | 3'UTR|outside_mRNA | ||
| Cre13.g580050 | (1 of 2) PTHR23074//PTHR23074:SF20 - AAA ATPASE // GH08677P | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGCACGTTTGCTGCTGCGCTGAGCCT |
| Internal bar code: | ACAAGCACGGTGCTGTAAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 953 |
| LEAP-Seq percent confirming: | 99.6783 |
| LEAP-Seq n confirming: | 4028 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCTGCTGTCTTCTAGGG |
| Suggested primer 2: | TGGTAAGTCCTGGCAGATCC |