Insertion junction: LMJ.RY0402.133850_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.133850
Chromosome:chromosome 2
Location:3707998
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095200 (1 of 3) PTHR19211:SF15 - ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGCAATCAAGCTCGCAGCTCAAGCCGA
Internal bar code:TGCGCGAGTGTCGCCTCCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:682
LEAP-Seq percent confirming:99.397
LEAP-Seq n confirming:3132
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTGACAATGGAGAGCCCC
Suggested primer 2:AAGGATGAATACCCGAACCC