| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133861 |
| Chromosome: | chromosome_7 |
| Location: | 4824081 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g345900 | DCL3 | Dicer-like protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAGGATGTGGGATGTGGGATTGGAGTCT |
| Internal bar code: | AGAGGGCTGGGAAGAGCTTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 79.5989 |
| LEAP-Seq n confirming: | 5080 |
| LEAP-Seq n nonconfirming: | 1302 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACCACTCCGTCCTTCAC |
| Suggested primer 2: | GAGGTGTGAGGACGGATGTT |