Insertion junction: LMJ.RY0402.134109_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.134109
Chromosome:chromosome 13
Location:2221998
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGTTGCATTGTATTACTCCTACCGCTCC
Internal bar code:AGGTGGCCCGGGGGGCGGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:252
LEAP-Seq percent confirming:99.1807
LEAP-Seq n confirming:4842
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACATACTCGCCTACACGCCT
Suggested primer 2:ACTGCAACCCATTCCTCAAC