Insertion junction: LMJ.RY0402.134289_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.134289
Chromosome:chromosome 6
Location:5550330
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g285250 LHCBM6 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chloropyll a/b binding protein of LHCII type I, chloroplast precursor CDS/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGGCCGAGGGGGTGAACTTGGTGGCGAA
Internal bar code:AGCTGACAACATCGAATGCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:855
LEAP-Seq percent confirming:99.1897
LEAP-Seq n confirming:1469
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGAAAAGGCTCTGCACC
Suggested primer 2:CTACTCTGAGAACGCGACCC