| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134413 |
| Chromosome: | chromosome 9 |
| Location: | 5833381 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402219 | LCI3 | Low-CO2-inducible protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCACCAGGCGGTGTCGGACAAACCCCGG |
| Internal bar code: | GTCTGGCTCGGCCAGTGTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 99.9369 |
| LEAP-Seq n confirming: | 3167 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGATCCACCAGTGTGCGTA |
| Suggested primer 2: | TTCGAGTGGCTGCTAAACCT |