| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134718 |
| Chromosome: | chromosome_13 |
| Location: | 2132805 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g577950 | VPS60 | Subunit of the ESCRT-III complex | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAATCACTCAGTCGCTCCTCAAACCCAGA |
| Internal bar code: | CGCTATCAACAAGGGGCATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 96.3115 |
| LEAP-Seq n confirming: | 1410 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGTGCTTGCGTATCAGA |
| Suggested primer 2: | CGCTGCACTGACTGCTACTC |