| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134722 |
| Chromosome: | chromosome 16 |
| Location: | 862597 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647950 | (1 of 33) IPR015797 - NUDIX hydrolase domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGGAGCAAATCCCGCCAGCAGCTTGGTT |
| Internal bar code: | GGCGCCAAGGATGCTGCACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.61 |
| LEAP-Seq n confirming: | 12004 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTCCTACCAGTACCCGC |
| Suggested primer 2: | ATAGCACGAAACTCCCATGC |