| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134895 |
| Chromosome: | chromosome 6 |
| Location: | 6351581 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292000 | (1 of 1) PF00790//PF03127 - VHS domain (VHS) // GAT domain (GAT) | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAGTGAAATTAAGTTTCAGGCCTCACTG |
| Internal bar code: | GTATGAGTCACGGCGGACGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 98.6281 |
| LEAP-Seq n confirming: | 2660 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCTTGCTCCCACAAGGTG |
| Suggested primer 2: | TCTCCAGAAGCTGAGGGTGT |