| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134917 |
| Chromosome: | chromosome 5 |
| Location: | 1041435 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246377 | ADA2 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.2 - Adenine deaminase / Adenine aminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGAGCGCCTAGCTCTGCTCCATTTCTC |
| Internal bar code: | AGCGGAATTCTTCGCCAGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 466 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGTAAAGCATTGGCTTG |
| Suggested primer 2: | TCCGCTCGTACTAGCTTGGT |