| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135240 |
| Chromosome: | chromosome 12 |
| Location: | 2063807 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509650 | PDS1,PDS | (1 of 1) K02293 - 15-cis-phytoene desaturase (PDS, crtP); phytoene desaturase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGAAGCGGGTAGGCAGGCAGGAGACAG |
| Internal bar code: | CTTGTTCTAGTCACTGTCCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.2017 |
| LEAP-Seq n confirming: | 6959 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCAAGGCTAGTTCAAGT |
| Suggested primer 2: | AACGTGTAGAGGCATTTGGG |