| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135320 |
| Chromosome: | chromosome 7 |
| Location: | 6202319 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g356350 | DXS1 | 1-deoxy-D-xylulose 5-phosphate synthase, chloroplast precursor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCACACGCACCGTGACCCATGAAATGG |
| Internal bar code: | GCTCTGTCCGCGCGGGCCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTTCACGCTGCGAATCA |
| Suggested primer 2: | TGACAAGAGTGGCAGACAGG |