| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136340 |
| Chromosome: | chromosome 8 |
| Location: | 549026 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359350 | CMP2,BC1, CARB1 | (1 of 1) K01961 - acetyl-CoA carboxylase, biotin carboxylase subunit (accC); Biotin carboxylase (ACCase complex) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATCAACACACAACCACCGCTATCACAT |
| Internal bar code: | CAGCGGATCAGCGCACGCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 99.7627 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCATGGTTTTCACACCT |
| Suggested primer 2: | TAACAGCCTCGTGCAGTGTC |