Insertion junction: LMJ.RY0402.137080_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.137080
Chromosome:chromosome 14
Location:1019997
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g614400 SRH23 (1 of 1) K15711 - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A3 [EC:3.6.4.- 6.3.2.19] (SMARCA3, HLTF); SNF2-related DNA/RNA helicase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCGCTCTGTGGGCTGGTTCTGGATCTT
Internal bar code:TCTTTCCGCGCTTCTTCCACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:183
LEAP-Seq percent confirming:98.5782
LEAP-Seq n confirming:208
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AATAAACGCACAGGGACAGG
Suggested primer 2:AAAGGGCCAGTACAACATGC