Insertion junction: LMJ.RY0402.137245_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.137245
Chromosome:chromosome 7
Location:4866883
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g346050 CRD1,CHL27A Copper response defect 1 protein; (1 of 2) 1.14.13.81 - Magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase / Mg-protoporphyrin IX monomethyl ester oxidative cyclase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTAATGAGCCCTGCCATAACTTCTCTTT
Internal bar code:TTAGGCGCGCTCTTTCGAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:933
LEAP-Seq percent confirming:97.4366
LEAP-Seq n confirming:10377
LEAP-Seq n nonconfirming:273
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGCTGACTGCGTTGACA
Suggested primer 2:CACCAATACAAATCCTGGGG