| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.137471 |
| Chromosome: | chromosome_10 |
| Location: | 4448526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g451600 | sense | CDS|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGACCCGACCTCTCACCGGCACTCTACGC |
| Internal bar code: | AACCGCCTAACAAGCGCGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 91.9091 |
| LEAP-Seq n confirming: | 214570 |
| LEAP-Seq n nonconfirming: | 18889 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCACGAATGTACAGGACT |
| Suggested primer 2: | GGACGGGAAGACGTGTTAAA |