Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.137841 |
Chromosome: | chromosome_12 |
Location: | 3050732 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g500550 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCTGATCGACCAGCTCACACCCGACGACT |
Internal bar code: | GCTAGTGTACGGGGACGAGGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 795 |
LEAP-Seq percent confirming: | 62.8421 |
LEAP-Seq n confirming: | 5143 |
LEAP-Seq n nonconfirming: | 3041 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGCCAACTTACTTCCAGGC |
Suggested primer 2: | GTTCCTGTCCTTTCTGCTGC |