| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138635 |
| Chromosome: | chromosome 12 |
| Location: | 1283695 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489150 | SHD1 | SH2-domain protein; (1 of 2) IPR000980 - SH2 domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGTTATCCGGTGGGTGTCATGAACAAT |
| Internal bar code: | CCGGGAGCAGGACATGGTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 34 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATTAGCGGCTTCGACAT |
| Suggested primer 2: | GGTTCGTTTTTATACCGCGA |