| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138691 |
| Chromosome: | chromosome 12 |
| Location: | 8309845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549800 | CSK5 | (1 of 3) K03097 - casein kinase II subunit alpha (CSNK2A); Casein kinase II-related Ser/Thr kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGTATGGTTGTAGACGGTTGAACTGTAT |
| Internal bar code: | TGTTGAGGTCATCGCGCTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 99.2841 |
| LEAP-Seq n confirming: | 9430 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTAGCGATGAAAGTGAT |
| Suggested primer 2: | GCCGACCTTACGTGTTGTTT |