| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138705 |
| Chromosome: | chromosome 10 |
| Location: | 2815204 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439350 | (1 of 3) PTHR17130:SF24 - GAN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCACCCCTCCCCCCCCCGCACCACCGCG |
| Internal bar code: | GTTCTGTTGATAGTAATGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 96.6953 |
| LEAP-Seq n confirming: | 3599 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGATAACTGAAACCCAGA |
| Suggested primer 2: | GTCGAAGTTGGGCACGTAGT |