Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.139156 |
Chromosome: | chromosome_9 |
Location: | 3828896 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g391801 | VSP4 | Hydroxyproline-rich glycoprotein, cell wall protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGCACTTGATTGCTTGGGAGATGAAGAGA |
Internal bar code: | CGGAGGACTCTTCTATGGGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 889 |
LEAP-Seq percent confirming: | 99.7146 |
LEAP-Seq n confirming: | 4193 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGAAAATGGGTGTCAGGT |
Suggested primer 2: | CAAGCAACCCGCTCTTCTAC |