| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139185 |
| Chromosome: | chromosome 3 |
| Location: | 5761706 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188500 | VPS46 | Subunit of the ESCRT-III complex; (1 of 1) K12197 - charged multivesicular body protein 1 (CHMP1, VPS46, DID2) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACGCTGTAACTCATCACGAAGCATGCG |
| Internal bar code: | GCGTGGTCGATCACTTCATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1263 |
| LEAP-Seq percent confirming: | 98.2314 |
| LEAP-Seq n confirming: | 14274 |
| LEAP-Seq n nonconfirming: | 257 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTGAGGTACAGGTGGAA |
| Suggested primer 2: | CTTCAGAACTTCACGAGGGC |