| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.139277 |
| Chromosome: | chromosome 5 |
| Location: | 2979195 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238332 | PSAD,PSAD1 | Photosystem I reaction center subunit II, 20 kDa; (1 of 1) K02692 - photosystem I subunit II (psaD) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAAATGGCCGTCATGATGCGCACCCAG |
| Internal bar code: | TTCAATGCCTAATGTTCCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 96.4429 |
| LEAP-Seq n confirming: | 6724 |
| LEAP-Seq n nonconfirming: | 248 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGATGGGGTTGACGTTCT |
| Suggested primer 2: | TCTCAGCATGCCTCAACAAC |