Insertion junction: LMJ.RY0402.139560_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.139560
Chromosome:chromosome 6
Location:8240513
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g305900 CPLD22,UAA4 Putative GDP-fucose transporter, conserved in the plant lineage and diatoms; (1 of 3) PTHR11132//PTHR11132:SF64 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCAAACCAAAGGTGTTATTCCATTCTG
Internal bar code:CCTGGTTTGGGCGCGCCCGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:841
LEAP-Seq percent confirming:99.7237
LEAP-Seq n confirming:27071
LEAP-Seq n nonconfirming:75
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAGGAACATTATGAGCAA
Suggested primer 2:TGTACAACCAGGAAGAGGGG