| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.139952 |
| Chromosome: | chromosome_3 |
| Location: | 3689354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g169250 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGCTTGCCCCCCAATGCACATCACAAGCC |
| Internal bar code: | TATGAGTCGGTTTCCGTACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 142 |
| LEAP-Seq percent confirming: | 99.8711 |
| LEAP-Seq n confirming: | 2325 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGACAGCATATCCTCCCC |
| Suggested primer 2: | TTTCTGTTTTCACGTGCTGC |