| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.140130 |
| Chromosome: | chromosome 13 |
| Location: | 3275519 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586050 | (1 of 1) PTHR13833:SF49 - NHL REPEAT-CONTAINING PROTEIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGACCCAGCCACCAAAAAGTGAGCGTG |
| Internal bar code: | GATGACAACGCGTATGTATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 86.6863 |
| LEAP-Seq n confirming: | 9135 |
| LEAP-Seq n nonconfirming: | 1403 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGTGTCTGACTCCCAA |
| Suggested primer 2: | AGGGTACCATACGGCAAGTG |