Insertion junction: LMJ.RY0402.140226_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.140226
Chromosome:chromosome 7
Location:1246087
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACGAAAACACTTGCATGGGGCGCACCCG
Internal bar code:CAAGTTTCTCCCCCCCGCACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:331
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACAGCGAGACTTTTCA
Suggested primer 2:AATAACGTTGTCTCCGTGGC