Insertion junction: LMJ.RY0402.140226_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.140226
Chromosome:chromosome 7
Location:1246088
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGTGCTCGTCAGTGCGGAGGCATGTCAT
Internal bar code:GCGTTATGCGGGGAGTGCAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:916
LEAP-Seq percent confirming:98.3841
LEAP-Seq n confirming:2679
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CCACACAGCGAGACTTTTCA
Suggested primer 2:AATAACGTTGTCTCCGTGGC