| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.140336 |
| Chromosome: | chromosome 13 |
| Location: | 1290754 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571050 | High mobility group protein; (1 of 1) PF00505//PF01388 - HMG (high mobility group) box (HMG_box) // ARID/BRIGHT DNA binding domain (ARID) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGGTCGGGATGGTGGTCGCAGAAGTA |
| Internal bar code: | ACGAACGGTCTAGACAGGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 99.6613 |
| LEAP-Seq n confirming: | 22657 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACAAACTGGCACCTTGG |
| Suggested primer 2: | ATCCCAAATGAAGAACTGCG |