Insertion junction: LMJ.RY0402.140417_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.140417
Chromosome:chromosome 4
Location:186794
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217000 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGGACTACGCAAACCTGTCCCTTAAGCC
Internal bar code:TCGTATGATTATCCGGTAGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAAATGTTTGGGGGTTTG
Suggested primer 2:ACAGTCCCAATTCAGTTCCG