Insertion junction: LMJ.RY0402.140852_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.140852
Chromosome:chromosome 14
Location:3735824
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632050 POB25,RPG1,RPGRIP1 Retinitis pigmentosa GTPase regulator-interacting protein 1; (1 of 1) PF11618 - First C2 domain of RPGR-interacting protein 1 (C2-C2_1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTAGAGAAGTCGAGCGGTCGGTCACAAC
Internal bar code:CAAAGTGACTCCTGGTCAACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:99.7112
LEAP-Seq n confirming:16915
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGTTTAATCTGGGCTGG
Suggested primer 2:CGGTACATCAAATGGCACAG