| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.141653 |
| Chromosome: | scaffold 24 |
| Location: | 73500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755647 | (1 of 1) K14404 - cleavage and polyadenylation specificity factor subunit 4 (CPSF4, YTH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCCCACACACACACACAGCGCTTTGC |
| Internal bar code: | GTCTTCGTTCGAGCTACGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 81.6727 |
| LEAP-Seq n confirming: | 18935 |
| LEAP-Seq n nonconfirming: | 4249 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTAGTGTCCACACGCT |
| Suggested primer 2: | ACCTGCCCCGTTAGTCTTTT |